Figure 2.
Mesenchymal stromal cells −1 and −2 represent the fibrosis-driving cells in ThPO-induced bone-marrow fibrosis
(A) Left panel: UMAP of non-hematopoietic cells from mice transplanted with thrombopoietin (ThPO; blue) or empty vector (EV; red; 5 and 10 weeks). Right panel: bar plot of cells per cluster (ThPO versus EV). Normalization to overall number of input per condition is shown. Fisher’s exact test with Bonferroni correction was used.
(B) Ridgeline plot comparing expression of NABA matrisome-associated gene sets (ThPO; blue versus control EV; red) condition. Competitive gene set enrichment analysis was used.
(C) Normalized differential gene expression (ThPO versus EV) of indicated genes in MSCs (Wilcoxon rank-sum test, two tailed).
(D) Overlap of the non-hematopoietic compartment and FACS Gli1+ cells in UMAP space.