Figure 4.

Mesenchymal stromal cells −1 and −2 are reprogrammed in JAK2^V617F-induced primary myelofibrosis

(A) Representative HE and reticulin staining of JAK2^V617F-induced fibrosis or control (EV, n = 4 mice per condition). Scale bar, 50 μm.

(B) Hemoglobin levels (mean ± SEM). Two-way ANOVA with post hoc pairwise t test was used.

(C) UMAP of non-hematopoietic BM cells (n = 1,292 cells).

(D) Top marker genes. Wilcoxon rank-sum test, p < 0.01. A full list of marker genes and corresponding GO terms is provided in Table S2.

(E) Bar plot of cells number in JAK2^V617F versus control (EV) after normalization to total number. Fisher’s exact test with Bonferroni correction was used.

(F) Volcano plot; Wilcoxon rank-sum test, two tailed; calculated per cluster between JAK2^V617F and JAK2^WT (top panel). Associated gene ontology terms (biological processes; BP) plotted semantically (bottom panel) are shown. (For a complete list of GO-terms and corresponding genes, see Table S3.)

(G) Ridgeline plot for matrisome gene sets in MSC and OLC clusters (JAK2^V617F; blue versus control; red) condition. Competitive gene set enrichment analysis was used.

(H) PROGENy analysis of MSC-1 and MSC-2. Sampling based permutation (10,000 permutations). Pathway activity scores as Z scores are shown.

See also Figure S4 and Tables S1, S2, S3, and S4.