f4 statistics involving Isthmian individuals, related to Figures 2 and 4
(A) f4 statistics in which Panamanian populations (W) were compared to Mixe (X), typically used to reveal UPopY among IA, and to four Australasian populations (Y). (B) Isthmian groups (W) were compared to each other (X) testing for other IA populations (Y, colored according to their geographic location) to test through the Z score whether a given Isthmian group carries excess of a specific IA ancestry. (C) To specifically test the differential relationships of Isthmian and other Central/South IA groups with Anzick-1 and Spirit Cave, we ran the f4 -statistics in the form f4 (Anzick1, Spirit Cave; Central and South IA, Mbuti) and reported the average Z score on a map. The Isthmian populations were also tested separately. In the lower part of the panel, we verified the same relationships in the form f4 (USR, Anzick1/Spirit Cave; Central and South IA, Mbuti), using USR as outgroup to the Central and South IA populations (again the Isthmian populations were tested separately, plot on the lower right). The datasets uIA89, mIA417 and ancient individuals were used considering different sets of variants.