Figure 4.

Full-length extension by hPol η, ɩ, and κ in the presence of all four dNTPs.A, 12-mer primer and 19-mer template DNA sequences, where X is dA or 15-mer peptide cross-linked at N⁶-dA. Reactions were done in the presence of: (B) 20 nM hPol η; (C) 40 nM hPol ɩ; and (D) 20 nM hPol κ. All experiments were done at 37 °C for 0, 5, 10, 20, 60, and 120 min (i.e., lanes, 1–6, 7–12, 13–18, 19–24, 25–30, 31–36). P, FAM-labeled 12-mer DNA primer.