Figure 5.

Single nucleotide insertion by hPol η, ɩ, and κ.A, 14-mer primer and 19-mer template sequences, where X is dA or N⁶-dA-peptide. Reactions were done in the presence of: (B) 5 nM hPol η; (C) 5 nM hPol ɩ; and (D) 5 nM hPol κ. All experiments were done at 37 °C for 0, 5, 10, and 30 min. Lanes 2–4 and 14–16, dATP; lanes 5–7 and 17–19, dTTP; lanes 8–10 and 20–22, dCTP; lanes 11–13 and 23–25, dGTP. P, FAM-labeled 14-mer DNA primer.