Table 2.
Primer: Template: |
5′-FAM-GGTGGTCCAUAA-3′ 3′-CCACCAGGTATTTGXCTCT -5′ |
|||
---|---|---|---|---|
X | Sequence | Yield (%) | Observed m/z | Base added |
dA | 5′-pAAACTGAGAG-3′ | 50 | 1053.9 (−3) | T, plus blunt end addition of A and G |
5′-pAAACTGAGAA-3′ | 50 | 1048.64 (−3) | ||
N6-dA-peptide | 5′-pAAACTGAGA-3′ | 50 | 944.27 (−3) | T |
5′-pAAACTGAGAG-3′ | 28 | 1053.82 (−3) | T, plus blunt end addition of A and G | |
5′-pAAACTGAGAA-3′ | 22 | 1048.64 (−3) |
The bold and underlined T is the base inserted opposite the adduct X.