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. 2021 Feb 20;296:100444. doi: 10.1016/j.jbc.2021.100444

Table 2.

Summary of products of extension of template–primer complexes by hPol η analyzed by LC-ESI-MS/MS

Primer:
Template:
5′-FAM-GGTGGTCCAUAA-3′
3′-CCACCAGGTATTTGXCTCT -5′
X Sequence Yield (%) Observed m/z Base added
dA 5′-pAAACTGAGAG-3′ 50 1053.9 (−3) T, plus blunt end addition of A and G
5′-pAAACTGAGAA-3′ 50 1048.64 (−3)
N6-dA-peptide 5′-pAAACTGAGA-3′ 50 944.27 (−3) T
5′-pAAACTGAGAG-3′ 28 1053.82 (−3) T, plus blunt end addition of A and G
5′-pAAACTGAGAA-3′ 22 1048.64 (−3)

The bold and underlined T is the base inserted opposite the adduct X.