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. 2021 Jan 19;11(5):1369–1385. doi: 10.1016/j.jcmgh.2021.01.007

Figure 1.

Figure 1

Increased TRIM21 expression is associated with HCC. (A) In the Gene Expression Omnibus Series, 6 studies showed elevated TRIM21 expression in HCC compared with normal liver tissues. (B) TCGA shows increased TRIM21 TPM in liver cancer comparing with normal tissues. (C)TRIM21 high expression indicates poor prognosis with decreasing overall survival according to TCGA data analysis, while the disease-free survival is trending lower yet not significant. (D) Liver tissue sections were stained for TRIM21 by immunohistochemistry (IHC). Representative TRIM21 staining on normal liver tissues from peripheral hepatic hemangioma (left panel; n = 20), on tumor tissues from HCC (middle panel; n = 49), and on paracancerous tissues 2 cm away from the HCC (right panel; n = 29). (E) TRIM21 expression is increased in HCC tissues compared with the paracancerous tissues and normal liver tissues. Relative intensity of staining in each section was assessed blindly and assigned a value ranging from 0 to 4 according to intensity of staining: score 0, negative staining, no positive cells; score 1, weak staining, the ratio of positive cells to total cells ≤25%; score 2, moderately positive, the ratio of positive cells to total cells ≤50%; score 3, moderately positive, the ratio of positive cells to total cells ≤75%; score 4, strongly positive, the ratio of positive cells to total cells>75%. Low expression defines the score from 0 to 2 and high expression is the score 3–4. (F) Receiver-operating characteristic (ROC) curves of the ability of TRIM21 or AFP high expression to the diagnosis of HCC. (G) TRIM21 was silenced in HCT116 and MHHC-97L human cells, or reconstituted with vector or HA-hTRIM21 in TRIM21–/– MEFs. Cells were lysed in RIPA buffer (1% SDS) and subjected to WB. The TRIM21 antibody obtained from Bioss (bs-0635R) or Proteintech (12018-1-AP) was used to detect human TRIM21. Note that the Bioss antibody fails to recognize human TRIM21, and neither antibody recognizes murine TRIM21. (H) TRIM21 antibody (Bioss bs-0635R) was used for IHC on HCC tumor tissues and paracancerous tissues collected 2 cm away from the HCC. Note that the Bioss antibody fails to detect difference in IHC staining in paracancerous and HCC tissues. ∗P < .05. ∗∗P < .01. ∗∗∗P < .001.