Figure 2.

Sox9^EGFPis expressed in intrahepatic bile ducts and peribiliary hepatocytes. Immunofluorescence demonstrates that EGFP is co-expressed in (A) 100% of EPCAM⁺ and (B) K19⁺ positive BECs across left, median, and right lobes. (C) Rare peribiliary cells expressing very low levels of EGFP co-localize with HNF4A and are morphologically consistent with hepatocytes (white arrows indicate EGFP⁺/HNF4A⁺; yellow arrows indicate EGFP⁺/HNF4A^–). (D) Sox9^EGFP does not co-localize with mesenchymal marker, vimentin (VIM) (scale bar = 50 μm).