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EGFP levels are not predictive of endogenous SOX9 levels. (A) Bisbenzimide and WGA label nuclei and cell membranes, respectively, for quantification of SOX9 and EGFP in single cells (roman numerals denote cells across multiple channels; scale bar = 25 μm). (B) SOX9 correlates poorly with EGFP levels (n = 747 cells). a.u. = arbitrary units.
