Figure 7.

BEC genes demonstrate differential expression across Sox9^EGFPpopulations. (A) Egfp expression is significantly different between GFP^{neg, sub, low,} and ^high populations. (B) RT-qPCR probes detecting Sox9 mRNA show enrichment between GFP^neg and ^sub populations but not GFP^low and ^high. Primers against nascent Sox9 RNA demonstrate up-regulation in GFP^high. (C) Canonical BEC biomarkers Krt19 and Spp1 are differentially expressed in Sox9^EGFP populations. (D) Cyr61 and Hes1 are most significantly enriched in GFP^high cells, suggesting increased Yap activity (letters indicate grouping by significance, P < .05, one-way analysis of variance and Tukey test).