Figure 9.

Functional differences in organoids derived from single GFP⁺cells are dependent on medium conditions. (A) Single cells from Sox9^EGFP populations form organoids in medium conditions developed for BECs (BEC media) and hepatocytes (TNFα media) (scale bar = 25 μm). (B and C) TNFα media significantly increase organoid formation relative to BEC media in all Sox9^EGFP populations and are required for significantly different organoid formation rates between GFP^sub, GFP^low, and GFP^high (B): letters indicate grouping by significance, P < .05, one-way analysis of variance and Tukey test; (C) ∗ indicates significance, P < .01, unpaired t test. (D and E) TNFα media also drive more significant increases in organoid size between 7 and 14 days of culture and result in significantly larger GFP^sub- and GFP^low-derived organoids at day 14 relative to GFP^high-derived organoids at the same time point (D): letters indicate grouping by significance, P < .05, one-way analysis of variance and Tukey test; (E): ∗ indicates significance, P < .002; ∗∗ indicates significance, P ≤ .0001, unpaired t test.