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. Author manuscript; available in PMC: 2021 Apr 7.
Published in final edited form as: J Invest Dermatol. 2018 Dec 27;139(6):1318–1328. doi: 10.1016/j.jid.2018.12.012

Figure 3. Triggering of TLR2 by Leishmania major LPGs induce neutrophil recruitment to the site of infection.

Figure 3.

(a, b) Primary WT and Tlr2−/− keratinocytes were exposed to L. major or L. major-purified LPG, and 24 hours later, cell-free supernatant was collected and tested for KC and MIP-2 content by ELISA. Representative mean values ± standard error of the mean from one of four experiments are shown. n ≥ 3. (c) Kinetics of neutrophil recruitment ± standard deviation in the ears of C57BL/6 mice after injection of WT, lpg1−/− (lpg11-knockout), and lpg1 AB L. major and (d) lpg2−/− (lpg2-knockout) and lpg2 AB L. major. (e) Kinetics of neutrophil recruitment ± standard deviation during the first day after infection in Tlr2−/− mice with the indicated L. major parasites. The data presented are representative of three independent experiments (n = 3–5 mice/group per time point) with similar results. *P < 0.05, **P < 0.01. AB, add back; LPG, lipophosphoglycan; TLR, toll-like receptor; WT, wild type