Figure 4. TLR2 signaling in nonhematopoietic cells is involved in early neutrophil recruitment.

(a) WT and TLR2 recipient mice were irradiated and reconstituted with bone marrow (BM) from WT or Tlr2^−/− donor mice as depicted. As a control, WT BM was transferred into irradiated WT mice. At 8 weeks later, BM-reconstituted mice were inoculated intradermally in the ear with 10⁶ Leishmania major organisms. (b) Representative gating strategy and (c, d) number ± standard deviation of Ly6G⁺ neutrophils recruited to the site of injection 24 hours after infection, as quantified by flow cytometry analysis. The data are representative of two independent experiments. (e, f) Two weeks after infection, the parasite burden ± standard deviation in the infected ears of the indicated chimeras was analyzed by limiting dilution analysis. The data are representative of two independent experiments including ≥3 mice/group. *P < 0.05, ***P < 0.001. TLR, toll-like receptor; WT, wild type.