Figure 4.

Total secreted proteins of Fov activate Ca²⁺ influx and trigger a hypersensitive response. a) Analysis of hypersensitive response triggered by coinfiltration of GhGLR4.8 and total Fov secreted proteins (SEPs) in Nicotiana benthamiana. GhGLR4.8^A and GhGLR4.8^C were expressed by Agrobacterium‐mediated transient transformation (Agro‐infiltration). For coinfiltration, tobacco leaves were infiltrated with SEPs 48 h after Agro‐infiltration. Cell death triggered by coinfiltration was visualized by trypan blue staining 60 h after Agro‐infiltration. Representative photographs are shown. b) Measurements of Ca²⁺ influx triggered by SEPs in cotton root meristem of Fov7 knockout and J668 seedlings by the scanning ion‐selective electrode method. AP5, an iGluRs antagonist. Error bars, mean ± SD, n = 4.