Figure 2.

Combination of EGCG and resveratrol is suitable for chemoprevention. (A) Tu212 cells were treated with the indicated doses of EGCG, resveratrol and their combination. Total cell lysates were used to assess the expression of PARP and cleaved caspase-3. Findings were confirmed by 2 or 3 repeats with almost similar experimental design (variation in dose and treatment time). Representative blots are presented. (B) Tu212 cells were treated with 30 µM EGCG, 15 µM resveratrol and their combination for two weeks. Plates were stained with 0.5% crystal violet. (C) MSK-Leuk1 (premalignant) cells were treated with different doses of EGCG, resveratrol and their combination for 72 h. Apoptosis was measured by Annexin V-PE staining from triplicate treatments. Error bars represent standard deviations. *P<0.05 indicates statistically significant increase in apoptosis when compared with the corresponding untreated control. EGCG, epigallocatechin gallate.