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. 2021 Mar 31;45(5):87. doi: 10.3892/or.2021.8038

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Copyright: © Amin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Inhibition of AKT-mTOR signaling by the combination of EGCG and resveratrol. (A) Tu212 and (B) SqCCy1 cells were treated with the indicated doses of EGCG, resveratrol or their combination for 24 h. Expression of p-AKT, p-mTOR, p-S6 and p-4E-BP1 was examined by western blotting in total cell lysates. Findings for A and B were confirmed by 2 or 3 repeats with almost similar experimental design (variation in dose and treatment time). Representative blots are presented. (C) Xenografted tumor tissues were stained for the expression of p-AKT, p-S6 and p-4E-BP1 by immunohistochemistry. Representative images from each group are presented. NT, no treatment; p, phosphorylated.