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. 2021 Mar 31;45(5):86. doi: 10.3892/or.2021.8037

Figure 5.

Figure 5.

Western blot analysis of colorectal cancer cell lysates treated with different concentrations of PRI for 24 h. (A) Phospho-mTOR and mTOR levels were increased and decreased respectively in HCT116wt and RKO cells carrying wild-type p53. However, there were no significant differences among the p53-mutant cell lines (HCT15 and DLD-1). Phospho-mTOR was normalized to total mTOR levels. PRI treatment also upregulated the expression of phospho-AMPK, and downregulated AMPK in HCT116wt, RKO and HCT15 cells. Phospho-AMPK was normalized to that of total AMPK and each experiment was performed in triplicate. Data are presented as the mean + SEM (n=3). **P<0.01, compared with the PRI untreated group. (B) Phospho-ULK1 and ULK1 expression was increased and decreased respectively in RKO, DLD-1 and HCT15 cell lines after PRI treatment. Phospho-ULK1 was normalized to total ULK1 levels. The expression of phospho-PI3K Class III was upregulated following PRI treatment in HCT116wt, RKO and HCT15 cells. Phospho-PI3K Class III was normalized to that of total PI3K Class III. Data are presented as the mean + SEM (n=3). **P<0.01, compared with the PRI untreated group. PRI, p53-reactivation and induction of massive apoptosis-1, APR-017 methylated; phosphor, phosphorylated; ULK1, Unc-51 like autophagy activating kinase 1.