Figure 5.

Stable knockdown of ZNRF2 potentiates paclitaxel (PTX) responsiveness in hsa-miR-105-delepted epithelial ovarian cancer (EOC) cells. (A) Establishment of EOC cells that were stably deprived of ZNRF2 expression was verified using western blot analysis. (B) Forty-eight hours after transfection with the indicated oligonucleotides, EOC cells were seeded in 96-well plates at the density of 5×10³ cells/well. Cells were then exposed to different doses of PTX as indicated or DMSO for 24 h, followed by measurement of cell viability using Cell Counting Kit-8 (*P<0.05, **P<0.01). (C) Anchorage-dependent clonogenic ability of EOC cells with different transfections was assessed using colony formation assay (*P<0.05, **P<0.01). ZNRF2, zinc and ring finger 2.