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Primer sequences for quantitative PCR of B. vulgatus and B. thetaiotaomicron
| Sequence | Target gene | Amplicon size (bp) | Reference | ||
|---|---|---|---|---|---|
| B. vulgatus | Forward | GCATCATGAGTCCGCATGTTC | 16s rRNA | 287 | Wu, 2010 [21] |
| Reverse | TCCATACCCGACTTTATTCCTT | ||||
| B. thetaiotaomicron | Forward | GCTTAATTTGCCTGTCTATCTCCT | α-1,6-mannanase | 75 | This study |
| Reverse | TGTTCCTTTCATCATTTGTCTCC |
