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. 2021 Apr 2;220(5):e202006040. doi: 10.1083/jcb.202006040

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© 2021 Götz et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 1. — Presynaptic proteins accumulate in rab2^−/−-deficient neuronal somata. (A) Confocal images of larval VNCs from wild-type and rab2^−/− mutants labeled for BRP (green). White squares indicate zoom area. (B) BRP sum intensity (wild-type: 100.0 ± 36.91%, n = 5; rab2^−/−: 1,701 ± 181.7%, n = 5). (C) Number of BRP aggregates (wild-type: 100.0 ± 40.93%, n = 5; rab2^−/−: 398.7 ± 35.27%, n = 5). (D–K) VNC (zooms) colabeled for BRP (green) and, in magenta, RIM-BP (D), VGlut (F), GFP-LAMP1 (H), and Cathepsin L (J; CathL). Number of RIM-BP aggregates (E; wild-type: 100.0 ± 30.77%, n = 8; rab2^−/−: 209.1 ± 39.04%, n = 8), VGlut aggregates (G; wild-type: 100.0 ± 20.22%, n = 8; rab2^−/−: 202.2 ± 27.43 5%, n = 8), LAMP1 aggregates (I; wild-type: 100.0 ± 36.72%, n = 5; rab2^−/−: 853.8 ± 133.1%, n = 5), and Cathepsin L aggregates (K; wild-type: 100.0 ± 10.32%, n = 5; rab2^−/−: 112.4 ± 11.36%, n = 5). (L) VNC from wild-type, rab2^−/− mutant, and rab2^−/− mutants with reexpressed Rab2 labeled for BRP (green). (M) Number of BRP aggregates (wild-type: 100.0 ± 58.90%, n = 5; rab2^−/−: 626.0 ± 96.90%, n = 4; Rab2 rescue: 227.4 ± 51.22%, n = 5). (N) BRP sum intensity (wild-type: 100.0 ± 53.28%, n = 5; rab2^−/−: 2,262.0 ± 308.3%, n = 4; Rab2 rescue: 461.0 ± 114.2%, n = 5). Scale bars: A and L, 10 µm; zoom (A, D, F, H, J, and L), 2 µm. All data provided as mean ± SEM. n represents single VNCs. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wt, wild-type.