Figure S2.

Stiff ECM enhances apoptosis in response to treatment. (A) Representative maximum-intensity projections and basal slice images of HCC70 cell cultured on soft and stiff substrates treated with DMSO vehicle stained with DAPI (gray). (B) Bar graph showing mean ± SEM of the percentage of HCC70 cells in contact with the basal substrate when cultured on soft (n = 4) or stiff (n = 4) substrates. (C) Representative images of MCF10A cells cultured on soft and stiff substrates and treated with 10 nM paclitaxel or DMSO vehicle stained for cleaved caspase 3 (green) and with DAPI (gray). (D) Bar graph showing mean ± SEM of loss of metabolic activity by MTT assay after IR treatment in MCF10A cells cultured on soft or stiff substrates (n = 2). (E) Representative images of HCC70 cells cultured on soft and stiff substrates and treated with 100 nM paclitaxel or DMSO vehicle stained for cleaved caspase 3 (green) and with DAPI (gray). All scale bars are 100 μm.