Figure 5.

Prediction of likely nitrogen, TORC1 or TORC2 dependencies for ‘non-cell-cycle-regulated’ proteins. (a) All significantly nitrogen and Torin1-regulated substrates were compared to published datasets, that included information about cell cycle regulation and substrates of mitotic kinases, to identify ‘non-cell-cycle-regulated’ proteins with fluctuating phosphorylation sites among our datasets. The Venn diagram compares the overlap of proteins with fluctuating phosphorylation sites from each perturbation. (b) The upper images illustrate the reasoning behind grouping proteins into three groups of potential: nitrogen-regulated, TORC1-dependent and TORC2-dependent regulations. All ‘non-cell-cycle’ substrates regulated by nitrogen (from nitrogen stress of wild-type, ssp2::ura4⁺ and cdc2.asM17) were grouped in the lower Venn diagram to compare these with all Torin1-regulated substrates.