Figure 8.

Functional validation for proteins likely to be regulated by nitrogen, TORC1 or TORC2. ‘Non-cell-cycle’ substrates belonging to the three group identified in figure 5b ((a) nitrogen-stress, (b) TORC1-dependent, (c) Torin1-dependent regulation) were compared to our published list of proteins for which the corresponding deletion strain which lacks the phosphorylated target is required for normal fitness in response to nitrogen stress or Torin1 treatment. The volcano plot illustrates significant change to phosphorylation greater than or equal to 2×, p ≤ 0.05 of ‘functional-validated’ proteins know to regulate fitness in response to nitrogen stress or following Torin1 addition to the growth media. The perturbation dataset in which each specific phosphorylation site was identified is highlighted according to the colours in the legend alongside identification of specific examples of specific proteins. Specific information for all sites are listed in table 3.