Table 3.
Functionally validated targets and specific information about their phosphorylation. ‘Non-cell-cycle-regulated’ molecules on which there is one or more sites at which phosphorylation levels fluctuate in response to an insult, for which the corresponding gene deletion strain shows altered fitness upon nitrogen stress or exposure to Torin1. Specific S. pombe IDs, gene names, phosphorylation site being regulated, product description and human orthologues are indicated. GO-term analysis has been used to identify the biological function that has been previously assigned to each molecule. Upward, or downward, pointing arrows indicate enhancement or reduction in phosphorylation respectively. The colour code indicates the specific perturbation for which fluctuation in phosphorylation at the site was identified; black, nitrogen stress of wild-type; blue, nitrogen stress of ssp2::ura4+; red, nitrogen stress of cdc2.asM17; green, Torin1 addition to wild-type. Sites identified in nitrogen stress cultures of: ↓ WT ↓ no Cdc2 ↓ AMPK or cultures added ↓ Torin1.
| proteins with altered fitness on minimal media or Torin1 that display fluctuating phosphorylation | ||||
|---|---|---|---|---|
| systematic ID | gene name | P-site | description | human orthologue |
| transmembrane transport | ||||
| SPCC663.03 | pmd1 | ↓S18 | leptomycin transmembrane transporter | ABCB1 |
| SPAC30D11.11 | izh3 | ↓S25 | ER membrane, transporter, zinc homeostasis (predicted) | PAQR3 |
| SPBC106.05c | tim11 | ↓T61 | F1-FO ATP synthase subunit E | ATP5ME |
| SPAC11G7.02 | pub1 | ↓S178 | HECT-type ubiquitin-protein ligase E3 | NEDD4 |
| chromatin organization | ||||
| SPAC664.03 | paf1 | ↑S384, S388 | RNA polymerase II-associated Paf1 complex | PAF1 |
| SPBC30B4.04c | sol1 | ↓S333, S342 | SWI/SNF complex | ARID1 |
| SPCC548.05c | dbl5 | ↑S182, S187, S280 | ubiquitin-protein ligase E3, degradation of histones | |
| TOR signalling | ||||
| SPBP18G5.03 | toc1 | ↓S399 | Tor complex Tor2 interacting protein 1 | |
| SPBC16E9.13 | ksp1 | ↓S378 | serine/threonine protein kinase | |
| SPAC630.13c | tsc2 | ↑T1034 | tuberin, GTPase activator | TSC2 |
| vesicle-mediated transport | ||||
| SPBC21D10.12 | hob1 | ↑S297, S300 | BAR adaptor protein Hob1 | BIN1 |
| SPBC31F10.16 | bch1 | ↓S445 | exomer complex ChAPs family | TTC17 |
| autophagy: SPBC28E12.06c | lvs1 | ↓S933 | beige protein homolog | WDFY3 |
| ribosomes: SPAC31G5.17c | rps1001 | ↓S135 | 40S ribosomal protein S10 | RPS10 |
| actin: SPCC1919.10c | myo52 | ↓S798 | myosin type V | MYO5 |
| FAD: SPCC1235.04c | nad1 | ↓S244 | FAD synthetase | FLAD1 |
| protein folding: SPBC1711.08 | aha1 | ↓S169 | chaperone activator | AHSA1 |
| lysine synthe: SPBC1105.02c | lys4 | ↑S497 | homocitrate synthase | |
| peroxisomes: SPCC550.09 | pex32 | ↓S198 | peroxin Pex32 (predicted) | |
| vitamins: SPAC1952.08c | SPAC1952.08c | ↓T188 | pyridoxamine 5'-phosphate oxidase (predicted) | |
| transcription: SPAC23E2.01 | Fep1 | ↑S231 up ↓S109 | iron-sensing transcription factor | |
| ?: SPAC56F8.02 | SPAC56F8.02 | ↑S116 | AMP binding enzyme, human DIP2 family (predicted) | DIP2A |