Fig. 3.
In vivo calcium imaging testing neuronal responses to i.a. injection of vehicle (7 μL, 0.1% BSA in PBS) or CCL2 (7 μL, #MJE00, R&D systems, 700 ng). a Percentage of neurons that responded to vehicle or CCL2 in naïve Pirt-GCaMP3 mice. Each dot = one mouse. n = 8; b Example ∆F/Fo plots are shown to depict how responses were determined. This neuron responded to CCL2 but not to BSA in a naïve Pirt-GCaMP3 mouse (this mouse had 0% vehicle responses and 1.5% CCL2 responses); Red line indicates the threshold for response = 4× standard deviation of the first 15 frames; Each dot indicates an imaging frame—the connecting line is included for ease of viewing peaks. c Percentage of neurons that responded to vehicle or CCL2 in naïve Ccr2 null × Pirt-GCaMP3 mice. n = 8; Mean ± SEM. Paired two-tailed t test, *p < 0.05. d Percentage of neurons that responded to CCL2 in naïve (n = 6) or DMM + 20 week (n = 8) NaV1.8 cre-GCaMP6s loxp mice. Unpaired two-tailed t test. *p < 0.05