Fig. 6.

Representative confocal images of the right knee of a 10-week-old male naïve Ccr2^RFP mouse, immunostained with CGRP. a CGRP/CCR2 overlay of the lateral compartment of the knee; b magnified white inset of a showing CCR2, CGRP signals and the overlay of both images. White arrows indicate an example of an overlap between CGRP and CCR2 signals (CCR2 receptors present on CGRP+ nerve fiber). c CGRP/CCR2 overlay of the medial compartment of the knee, an area where we do not see a high amount of innervation in naïve mice. Scale bar for a, c = 100 μm. Scale bar for b = 25 μm