Fig. 3.

Restriction fragment length polymorphism (RFLP) patterns of PCR products amplified from the DNA of Paragonimus metacercariae from Sawagani. The internal transcribed spacer 2 region of nuclear ribosomal DNA was amplified using PCR (lane 1) and treated with the restriction enzyme SnaBI (lane 2). The mitochondrial 16S ribosomal RNA gene region was amplified (lane 3) and treated with the restriction enzyme BsrDI (lane 4). A 100-bp DNA ladder marker was used to estimate the size of fragments (lane 5). Based on PCR-RFLP patterns, we identified metacercariae as the triploid form of Paragonimus westermani. Metacercariae from Mokuzugani exhibited the identical cleavage pattern as those from Sawagani.