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. 2021 Mar 26;23(6):401. doi: 10.3892/mmr.2021.12040

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Copyright: © Sheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Paeonol inhibits the migration and invasion of HeLa cells. (A) HeLa cells and (B) H8 cells were treated with different concentrations of Pae, and the cell viability at 24, 48 and 72 h was estimated via Cell Counting Kit-8 assay. (C and D) Colony-formation assay was performed to determine the colony-forming capacity of HeLa cells. (E and F) Wound healing assay was conducted to evaluate the migratory capacity of HeLa cells (magnification, ×100). (G and H) Transwell assay was performed to assess the invasive capacity of HeLa cells (magnification, ×100). (I) The protein levels of MMP-2, MMP-9 in HeLa cells were detected by western blot. *P<0.05, **P<0.01, ***P<0.001 vs. control group. MMP, matrix metalloprotease.