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. 2021 Mar 26;23(6):401. doi: 10.3892/mmr.2021.12040

Figure 4.

Figure 4.

5-LO inhibits Pae from exerting the anti-migratory and anti-invasive effects. (A) The cell viability at 24, 48 and 72 h was estimated via Cell Counting Kit-8 assay in HeLa cells divided into control, Pae, Pae+vector and Pae+pcDNA 3.1–5-LO groups. (B and C) Colony-formation assay was performed to determine the colony-forming capacity of HeLa cells in different groups. (D and E) Wound-healing assay was conducted to evaluate the migratory capacity of HeLa cells (magnification, ×100). (F and G) Transwell assay was performed to assess the invasive capacity of HeLa cells (magnification, ×100). (H) The protein levels of MMP-2, MMP-9 in HeLa cells were detected by western blotting. **P<0.01, ***P<0.01 vs. control group. #P<0.05, ##P<0.01, ###P<0.001 vs. Pae+vector group. 5-LO, 5-lipoxygease; MMP, matrix metalloprotease.