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. 2021 Mar 30;23(6):414. doi: 10.3892/mmr.2021.12053

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Copyright: © Tian et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — YAP OE reverses dioscin-mediated inhibition of cell proliferation. Effects of YAP OE and dioscin treatment on the cell cycle distribution were (A) determined via flow cytometry and (B) quantified. Effects of YAP OE and dioscin treatment on cell apoptosis were (C) determined via flow cytometry and (D) quantified. Cell proliferation was (E) detected by performing EdU staining (magnification, ×200) and (F) quantified. (G) Cell proliferation was also assessed by performing Cell Counting Kit-8 assays. (H) Protein expression levels were measured via western blotting. Data are presented as the mean ± SD of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001. YAP, yes-associated protein; OE, overexpression; NC, negative control; OD, optical density; ns, not significant; EdU, 5-ethynyl-2′-deoxyuridine.