Fig. 12.

In-vitro validation of anti-SARS-CoV2 activity: Vero E6 cells were in infected with SARS-CoV2 at 0.1 MOI for 2 h. Subsequently the virus inoculum is replaced with fresh media containing the 0.1% of vehicle (DMSO) or indicated concentration (0.01, 0.1, 0.5, 1, 2, 5, 10 μM) of various drugs. 24 h post treatment the viral load in the cells (cell associated) or supernatant (secreted virus) was determined using real-time PCR as described in methods. IC₅₀ values were determined from the dose response curve analysis (GraphPad prism). Vybrant MTT cell viability assay was used to determine cytotoxicity at low–high concentrations (0.01, 0.1, 0.5, 1, 5, 10 & 20 μM). The percentage cell viability was calculated with respect to vehicle treated control and 50% cytotoxic concentration (CC₅₀) was determined by dose–response curve (GraphPad prism). Experiments were done in duplicates.