Figure 3.

The isotopic selectivity of H₂-driven NADH generation from NAD⁺ by Bio, Chemo-Bio, and Chemo catalysts in the presence of ^xH₂O and ^xH₂. Analogous experiments were performed in varying mixtures of ¹H₂O and ²H₂O, under either (left) ¹H₂ or (right) ²H₂ gas. Lines of best fit through data for the Bio system are shown (green line: slope = 0.94, R² = 0.98 for each). The percent isotopic selectivity toward ²H incorporation into the [4]-position of NADH was determined using ¹H NMR spectroscopy as described in Section S.1.2.2 in the Supporting Information. Experimental details are provided in Section S.1.2.1 in the Supporting Information.