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. Author manuscript; available in PMC: 2021 Apr 7.
Published in final edited form as: J Med Chem. 2019 Mar 29;62(7):3524–3538. doi: 10.1021/acs.jmedchem.8b02009

Figure 3.

Figure 3.

Time-dependent human tumor cell-to-plasma partitioning of 6 and DON (1) release. 6 was incubated in P493B lymphoma cells suspended in human plasma, and at 0.25, 0.5, 1, and 2 h, aliquots were removed and evaluated for 6 and DON released from 6 using liquid chromatography (LC)–mass spectrometry (MS). (A) In human plasma, 6 was stable over the 2 h incubation period with minimal DON released. (B) In contrast, 6 partitioned into P493B tumor cells and was biotransformed to DON. These results confirm selective uptake and bioactivation of 6 to DON in tumor cells over 2 h.