FIGURE 7.

Binding of RNA and DNA fragments to PAI-1.A, biotinylated RNA or DNA was sonicated for the indicated times, subjected to agarose gel electrophoresis, and visualized by ethidium bromide staining. B, after sonication, the same RNA (black bars) or DNA (open bars) samples were added to wells of a microtiter plate coated with wild type PAI-1 and incubated for 2 h at room temperature. Bound nucleic acids were detected using peroxidase-labeled streptavidin and TMB as substrate. Data represent mean ± S.D. (n = 8) of a typical experiment of three. **, p < 0.0001; ***, p < 0.00001 versus nonsonicated RNA or DNA.