FIGURE 10.

Nuciferine regulated the expression of lipid-metabolism-related genes and promoted the expression of adipokines in fully differentiated human primary adipocytes. The human primary preadipocytes were plated into 24-wells plates at a density of 2 × 10⁴/well, 48 h after complete confluence, the cells were differentiated as described in the “Methods” section. On the ninth day of differentiation, the fully differentiated adipocytes were exposed to 0, 2.5, 5, 10, 20 μM nuciferine for 48 h. Then the total RNA was extracted and RT-qPCR were conducted to determine the mRNA levels of FAS (A), ACC (B), SREBP1 (C) HSL (D), FGF21 (E) and ZAG (F). All of the results were normalized to the values of the internal control PPIA, and the results were expressed as fold changes of the Ct value relative to the control value, which was defined as 1. All results were presented as mean ± SE in three independent experiments. *p < 0.05 vs the control group (0 μM).