FIGURE 2.
Nuciferine inhibited the proliferation of 3T3-L1 preadipocyte. 3T3-L1 preadipocytes were plated into 96-well plates at a density of 1×103 cells per well. 24 h later, the medium was refreshed and supplemented with different concentrations of nuciferine (0, 2.5, 5, 10 and 20 μM) for different action times (24, 48, 72, 96 and 120 h). Cell proliferation was determined by CCK-8 and the absorbance (A) were measured at 450 nm by spectrophotometer. Relative cell viabilities (B) were then calculated as the ratio of viable cells against controls (0 μM). Results are presented with mean ± SE from three independent experiments. OD, optical density. (A). p < 0.05 2.5 μM vs. the controls (0 μM), (B). p < 0.05 5 μM vs the controls (0 μM), (C). p < 0.05 10 μM vs the controls (0 μM), (D). p < 0.05 20 μM vs the controls (0 μM).