KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-K4m1 antibody | Abcam | ab8895; RRID: AB_306847 |
| Anti-K4m3 antibody | Abcam | ab8580; RRID:AB_306649 |
| Anti-K27m3 antibody | Millipore | 07-449; RRID:AB_310624 |
| Anti-p300 antibody | Santa Cruz Biotechnology | sc-585; RRID:AB_2231120 |
| Anti-STAT3 antibody | Thermo Fisher Scientific | 14-6727-81; AB_468247 |
| anti-CD3 antibody | Thermo Fisher Scientific | 16-0031-86; RRID:AB_468849 |
| anti-CD3 antibody | BioxCell | BE0001-1; RRID:AB_1107634 |
| anti-CD28 antibody | Thermo Fisher Scientific | 16-0281-86; RRID:AB_468923 |
| anti-CD28 antibody | BioxCell | BE0015-1; RRID:AB_1107624 |
| anti-IFN-γ antibody | BioXCell | BE0055; RRID:AB_1107694 |
| anti-IL-4 antibody | BioXCell | BE0045; RRID:AB_1107707 |
| anti-CD4 antibody | BD Biosciences | 550954; RRID:AB_393977 |
| anti-CD8 antibody | BD Biosciences | 551162; RRID:AB_394081 |
| anti-CD25 antibody | Thermo Fisher Scientific | 17-0251-81; RRID:AB_469365 |
| anti-CD44 antibody | Thermo Fisher Scientific | 12-0441-81; RRID:AB_465663 |
| anti-CD45.1 antibody | BD Biosciences | 17-0453-81; RRID:AB_469397 |
| anti-CD45.2 antibody | BioLegend | 48-0454-80; RRID:AB_11039533 |
| anti-CD62L antibody | Thermo Fisher Scientific | 25-0621-81; RRID:AB_469632 |
| anti-c-Kit antibody | BD Biosciences | 553354; RRID:AB_394805 |
| anti-Sca-1 antibody | BioLegend | 108112; RRID:AB_313349 |
| anti-Nkp46antibody | Thermo Fisher Scientific | 46-3351-82; RRID:AB_1834441 |
| anti-RORgt antibody | BD Biosciences | 562607; RRID:AB_11153137 |
| anti-T-bet antibody | Thermo Fisher Scientific | 25-5825-82; RRID:AB_11042699 |
| anti-Foxp3 antibody | Thermo Fisher Scientific | 48-5773-82; RRID:AB_1518812 |
| anti-IL-17A antibody | BioLegend | 506922; RRID:AB_2125010 |
| anti-IFN-γ antibody | Thermo Fisher Scientific | 17-7311-81; RRID:AB_469503 |
| anti-IL-4 antibody | BioLegend | 504120; RRID:AB_2562102 |
| anti-IL-13 antibody | Thermo Fisher Scientific | 50-7133-82; RRID:AB_2574279 |
| anti-TCR γ/δ antibody | BioLegend | 118118; RRID:AB_10612756 |
| anti-IL-2 antibody | BioxCell | BE0043-1; RRID:AB_1107705 |
| anti-c-MAF Monoclonal Antibody (sym0F1), PE | Thermo Fisher Scientific | Cat # 12-9855-42; RRID:AB_2572747 |
| Prime Flow c-Maf probe (Assay ID: VB6-14037-PF), Alexa Fluor 750 | Thermo Fisher Scientific | Cat # PF-204 |
| Prime Flow Il23r probe (Assay ID: VB1-17154-PF), Alexa Fluor 647 | Thermo Fisher Scientific | Cat # PF-204 |
| PrimeFlow™ RNA Assay Kit, 40 tests | Thermo Fisher Scientific | Cat # 88-18005-204 |
| Bacterial and Virus Strains | ||
| N/A | ||
| Biological Samples | ||
| N/A | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant Human TGF-β1 | R&D Systems | 240-B-010 |
| Recombinant Mouse IL-1 beta | R&D Systems | 401-ML-005 |
| Recombinant Mouse IL-12 | R&D Systems | 419-ML-010 |
| Recombinant Mouse IL-23 | R&D Systems | 1887-ML-010 |
| Recombinant Mouse IL-6 | R&D Systems | 406-ML-005 |
| Human IL-2 | National Cancer Institute | N/A |
| Dextran sulfate sodium salt | MP Biomedicals | 160110 |
| Critical Commercial Assays | ||
| TruSeq RNA Sample Prep Kit v2 | Illumina | RS-122-2001 |
| TruSeq Small RNA Sample Prep Kit | illumina | RS-200-0012 |
| Luc-Pair Duo-Luciferase Assay Kits 2.0 | GeneCopoeia | LF-001 |
| Lipofectamine LTX with Plus Reagent | Thermo Fisher Scientific | A12621 |
| TaqMan Reverse Transcription Kit | Thermo Fisher Scientific | 4366597 |
| TaqMan® Universal PCR Master Mix, No AmpErase® UNG | Thermo Fisher Scientific | 4364341 |
| TRIzol | Thermo Fisher Scientific | 15596018 |
| mirVana miRNA Isolation Kit | Thermo Fisher Scientific | AM1560 |
| Chromium Single Cell 3’ Reagent kits v2 | 10x Genomics | PN-120237 |
| Zombie NIR Fixable Viability kit | Biolegend | Cat #: 423105 |
| Deposited Data | ||
| Raw and analyzed data | This paper | GEO: GSE160250 |
| ChIP-seq samples for p300 and K4me1 (Th1) | Vahedi G, et al., 2012 | GEO: GSE40463 |
| ChIP-seq samples for STAT4 (Th1) | Wei L, et al., 2010 | GEO: GSE22104 |
| ChIP-seq samples for STAT3 and K27me3 (Th17) | Ghoreschi K, et al., 2010 | GEO: GSE23681 |
| ChIP-seq samples for calling super enhancers (Th cells) | Hnisz D, et al., 2013 | GEO: GSE17312 |
| Consensus coding sequence | Pruitt KD, et al., 2009 | Genome Res. 2009 Jul; 19(7): 1316–1323. |
| Experimental Models: Cell Lines | ||
| HEK293T | ATCC | CRL-3216 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J (WT) | The Jackson Laboratory | #000664 |
| B6.SJL-Ptprca/BoyAiTac (CD45.1+) | Taconic | #4007 |
| C57BL/6-Il17atm1Bcgen/J (Il17a-GFP) | The Jackson Laboratory | #018472 |
| C.B6 (Cg)-Rag2tm1.1Cgn/J (Rag2−/−) | The Jackson Laboratory | #008448 |
| B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ | The Jackson Laboratory | # 017336 |
| CD4-Cre-STAT3fl/fl | Dr. David E. Levy (NYU) | N/A |
| STAT4 KO | Dr. Mark Kaplan (IU) | N/A |
| C57BL/6-Mir221/222 fl/fl | This paper | N/A |
| Mir221/222 KO | This paper | N/A |
| CD4-Cre-mir221/222 fl/fl (conditional KO: cKO) | This paper | N/A |
| Rag2-miR-221/222 (double KO: DKO) | This paper | N/A |
| IL17-GFP miR-221/222 KO | This paper | N/A |
| Oligonucleotides | ||
| TaqMan® miRNA Assay (snoRNA202) | Thermo Fisher Scientific | 4427975-001232 |
| TaqMan® miRNA Assay (hsa-miR-221) | Thermo Fisher Scientific | 4427975-000524 |
| TaqMan® miRNA Assay (hsa-miR-222) | Thermo Fisher Scientific | 4427975-002276 |
| miRIDIAN microRNA Mimic (mmu-miR-221-3p) | Dharmacon | C-310583-07-0005 |
| miRIDIAN microRNA Mimic (mmu-miR-222-3p) | Dharmacon | C-310584-07-0005 |
| miRIDIAN microRNA Mimic Negative Control | Dharmacon | CN-001000-01-05 |
| Recombinant DNA | ||
| miRNA 3' UTR target clones for Maf | GeneCopoeia | MmiT024436-MT06 |
| miRNA 3' UTR target clones for Il23r | GeneCopoeia | MmiT038074-MT06 |
| miRNA Target clone control vector for pEZX-MT06 | GeneCopoeia | CmiT000001-MT06 |
| Software and Algorithms | ||
| BEDTOOLS | Quinlan and Hall, 2010 | http://bedtools.readthedocs.io/en/latest/index.html |
| bedGraphToBigWig | Kent et al., 2010 | https://genome.ucsc.edu/goldenpath/help/bigWig.html |
| wigToBigWig | Kent et al., 2010 | https://genome.ucsc.edu/goldenpath/help/bigWig.html |
| The Integrative Genomics Viewer (IGV) | Thorvaldsdottir et al., 2013 | http://software.broadinstitute.org/software/igv/ |
| Bowtie v1.1.2 | Langmead et al., 2009 | http://bowtie-bio.sourceforge.net/index.shtml |
| Cufflinks v 2.2.1 | Trapnell et al., 2012 | https://github.com/cole-trapnell-lab/cufflinks |
| MACS v 1.4.3 | Y. Zhang et al., 2008 | http://liulab.dfci.harvard.edu/MACS/index.html |
| Seurat R package 3.1.5 | Satija et al., 2015 | http://satijalab.org/seurat/ |
| R studio | RStudio | https://www.rstudio.com/ |
| Partek Genome Suite v6.6 | Partek Incorporated. | http://www.partek.com/ |
| Prism software | GraphPad | RRID: SCR_002798 |
| Cytoscape 3.6.0 | Nepusz et al., 2012 | RRID: SCR_003032 |
| ClueGO 2.5.0 | Bindea et al., 2009 | RRID: SCR_005748 |
| FlowJo v 9, v 10 | BD Biosciences | RRID: SCR_008520 |
| Other | ||
| NGS data generated in this study | GEO | GSE160250 |