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. 2021 Feb 24;13(3):298. doi: 10.3390/pharmaceutics13030298

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Band shift assay of the Fluc-mRNA nanocomplexes with (A) CS-SeNPs, (B) LA-CS-SeNPs, (C) PEG-CS-SeNPs, and (D) LA-PEG-CS-SeNPs, using a 2% agarose gel. Arrows indicate optimal binding ratios. Lane 1: Control (naked mRNA (0.3 µg/µL)). Lanes 2–8 (A) mRNA:CS-SeNP ^w/_w ratios (1:0.5; 1:1; 1:2; 1:3; 1:4; 1:5 and 1:5.5), (B) mRNA:LA-CS-SeNP ^w/_w ratios (1:6; 1:7; 1:8; 1:9; 1:10, 1:11 and 1:12), (C) mRNA:PEG-CS-SeNP ^w/_w ratios (1:1; 1:2; 1:3; 1:4; 1:5; 1:6 and 1:7), and mRNA PEG-LA-CS-SeNP ^w/_w ratios (1:1; 1:2; 1:3; 1:4; 1:5; 1:6 and 1:7).