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. Author manuscript; available in PMC: 2021 Apr 7.
Published in final edited form as: Cell Rep. 2020 Dec 29;33(13):108553. doi: 10.1016/j.celrep.2020.108553

Figure 2. Dynamic Features of Tracheal Myeloid Populations after Injury.

Figure 2.

(A) UMAP visualization of all SCRNA-seq data across airway injury and repair overlaid (3 animals pooled into 1 sample for each); captured cells: ctrl = 458, 1 dpP = 1,625, 4 dpP = 1,507, 7 dpP = 1,717.

(B) UMAP visualization of SCRNA-seq data on indicated days post-injury.

(C) Violin plots of M2-like macrophage gene signature, Arg1 and Arg2.

(D) Violin plots of classic monocyte/macrophage gene signature, Ccr2 and Cx3cr1.

(E) Violin plots of cell cycle genes associated with G2/M and S phase.

(F) Representative FACS plot and quantification of YARG expression gated on CD45+;F4/80+ (n = 3 mice per time point).

(G) FACS analysis and quantification of Ccr2-CreERT2;ROSA-tdTom+ cells from YARG animals after tamoxifen administration and sham or polidocanol treatment (n = 3, mice per time point).

(H) Immunofluorescence analysis of Ccr2-CreERT2;ROSA-tdTom animals; Krt5 (basal cells, green), Ccr2-CreERT2;ROSA-tdTom lineage trace (red), and Höechst nuclear stain (blue); scale bar, 15 μm.

(I) Histology of wild-type animals; Krt5 (red), CD45 (green), nuclear stain Höechst, scale bar, 15 μm.

(J) Confocal image of peeled epithelial sheet showing F4/80+ myeloid cells (green) and Krt5+ epithelial basal cells (red), Höechst nuclear stain. See also Figure S2 and Tables S2 and S4.