Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 7;7(15):eabc6747. doi: 10.1126/sciadv.abc6747

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

PMC Copyright notice

Fig. 4 — (A) Experimental approach to detect germ cell cytoplasmic sharing using recombination-induced multicolor clonal labeling. (B) Schematized formation of bicolored germ cell clones with R26R-Rainbow;Pou5f1Cre-ER in the presence and absence of ICBs through cytoplasmic sharing of FPs in WT and Tex14^−/− genetic backgrounds, respectively. (C) Endogenous expression of FPs in E13.5 R26R-Rainbow;Pou5f1Cre-ER;Tex14^+/+ and R26R-Rainbow;Pou5f1Cre-ER;Tex14^−/− ovaries reveals divergent clone organization. Magnified image below shows a bicolor clone positive for mCerulean and mOrange in a R26R-Rainbow;Pou5f1Cre-ER;Tex14^+/+ E13.5 ovary. Although germ cells expressing different FPs were closely juxtaposed in some cases, bicolored clones were not observed in E13.5 R26R-Rainbow;Pou5f1Cre-ER;Tex14^−/− ovaries. (D) The mean clone size in 214 clones from E13.5 R26R-Rainbow;Pou5f1Cre-ER;Tex14^+/+ ovaries was 8 (mCerulean, 64 clones; mOrange, 33 clones; mCherry, 177 clones). (E) Table of total labeled cells, estimated total labeled clones (based on mean clone size of 8), total bicolored clones, and estimated percentage of bicolored clones in R26R-Rainbow;Pou5f1Cre-ER;Tex14^+/+ (n = 3) and R26R-Rainbow;Pou5f1Cre-ER;Tex14^−/− (n = 4) ovaries at E13.5 (see fig. S4D for the breakdown of total number of cells per genotype).