Figure 2. The majority of the PD-L1⁺ cells within the TME are TAMs.

(A) Percentages of CD11b^–, CD11b⁺F4/80^–, and CD11b⁺F4/80⁺ cells within PD-L1⁺ population were assessed with FACS. (B) Immunofluorescence staining of PD-L1 (shown in green) and F4/80 (shown in red) in subcutaneously inoculated Hepa1-6 tumors. Scale bar: 100 μm. Values are mean ± SEM of a minimum of 3 independent experiments. n = 5 biological replicates per group (A and B).