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. 2021 Mar 22;6(6):e147282. doi: 10.1172/jci.insight.147282

Figure 1. The CCL2-CCR2 axis is necessary for recipient classical monocytes (CM) recruitment to the allograft.

Figure 1

(A) Flow cytometry quantification of CM (live CD45+Ly6G–NK1.1–CD11b+SiglecF–CD24–Ly6Chi) recruited into the allograft after treatment of recipients with i.v. IgG isotype or anti-CCR2 antibodies (n = 5). (B) Flow cytometry quantification of CM as described in A, recruited into the allograft using WT or Ccr2–/– recipient mice (n = 5). (C) Flow cytometry quantification of CM (live CD45+Ly6G–NK1.1–CD19–CD11b+CF4/80–CD11c–Ly6Chi) in spleens of WT or Ccr2–/– recipients after lung transplant (n = 5–6). (D) Flow cytometry quantification of CM as described in A, recruited into the allograft after treatment of recipients with i.v. IgG isotype or anti-CCL2 antibodies in recipient mice (n = 3). Graphs show means ± SD. Graphs were analyzed by unpaired Student’s t test. ***P < 0.001; ****P < 0.0001.