Figure 7. Aged lung Treg cells fail to upregulate a prorepair transcriptional program to the same extent as young Treg cells during recovery from influenza infection.

(A) MA plot comparing gene expression of young Treg cells during the naive state with young Treg cells during the recovery phase of influenza infection. Genes of interest are annotated and y axis denotes fold-change dynamic range. (B) MA plot comparing gene expression of aged Treg cells during the naive state with aged Treg cells during the recovery phase of influenza infection. Genes of interest are annotated and y axis denotes fold-change dynamic range. Note that the y axis extends to +8 in A and +5 in B. (C and D) GSEA dot plot results highlighting key statistics (FDR q value and normalized enrichment score or NES) and enriched gene sets per phenotype. Genes were ordered by log₂(fold change) and ranked by the young Treg cell–influenza (C) or aged Treg cell–influenza (D) phenotype. Red dots denote gene sets with a positive enrichment score or enrichment at the top of the ranked list. Blue dots denote gene sets with a negative enrichment score or enrichment at the bottom of the ranked list. (E and F) Top 3 GSEA positive enrichment plots for the young Treg cell–influenza (E) or aged Treg cell–influenza (F) phenotype. n = 5 mice/group (young — naive, aged — naive, young — influenza, and aged — influenza) for all panels.