Figure 2. Defining the downstream projections of LepRb^VMH neurons.

(a) Experimental schematic showing the injection of the Driver AAV with the Payload AAV encoding a GFP-2A-SynmRuby transgene into the VMH of Slc17a6^FlpO;Lepr^Cre animals. (b,c) Representative image of GFP-IR (green) showing viral transduction in the VMH (b) and projections (c) in the periaqueductal gray (PAG), arcuate nucleus (ARC), paraventricular hypothalamic nucleus (PVN), bed nucleus of the stria terminalis (BNST), and preoptic area (POA). (d) Cartoon showing the projection targets of LepRb^VMH neurons.