Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-FOS (Rabbit monoclonal) | Cell Signaling | Cat# 2250; RRID:AB_2247211 | (1:1000) |
| Antibody | Anti-GFP (Chicken polyclonal) | Aves Laboratories | Cat# GFP1020; RRID:AB_10000240 | (1:1000) |
| Antibody | Anti-dsRed (Rabbit polyclonal) | Takara | Cat # 632496; RRID:AB_10013483 | (1:1000) |
| Cell line (E. coli) | Stbl3 E. coli | Thermofisher | Cat # C737303 | |
| Recombinant DNA reagent | pAAV-nEF Con/Fon hChR2(H134R)-EYFP | Fenno et al., 2014; Addgene | Addgene Plasmid ID:55644 | |
| Recombinant DNA reagent | AAV-hSYN1-fDIO-tTA | This manuscript | Addgene plasmid ID: 166597 | hSYN1-driven, Flp-dependent expression of tTA |
| Recombinant DNA reagent | AAV-TRE-DIO-hM3Dq-mCherry (Cre-ON) | This manuscript | Addgene plasmid ID: 166599 | TRE-driven, Cre-dependent expression of hM3Dq-mCherry |
| Recombinant DNA reagent | AAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF) | This manuscript | Addgene plasmid ID: 166609 | TRE-driven, Cre-dependent inactivation of hM3Dq-mCherry |
| Recombinant DNA reagent | AAV-TRE-DIO-ChR2-Tdtomato | This manuscript | Addgene plasmid ID: 166600 | Cre-dependent expression of ChR2-TdTomato downstream of a TRE |
| Recombinant DNA reagent | AAV-TRE-DIO-GFP-2A-SynmRuby | This manuscript | Addgene plasmid ID:166608 | Cre-dependent expression of GFP-2A-Synaptophysin-mRuby downstream of a TRE |
| Chemical compound, drug | CNO | Tocris | Cat# 4936 | |
| Genetic reagent (M. musculus) | LeprIRES-Cre | Jackson Laboratories | Strain #: 032457 | Mouse: Lepr-IRES-Cre |
| Genetic reagent (M. musculus) | Slc17a6IRES-FlpO | This manuscript | N/A | Slc17a6 FlpO knockin mouse |
| Genetic reagent (M. musculus) | C57Bl6J | Jackson Laboratories | Strain #: 000664 | Wild-type animals |
| Genetic reagent (M. musculus) | Rosa26LSL-eGFP-L10a | Krashes et al., 2014 | N/A | Rosa26 targeted Cre-dependent eGFP_L10a allele |
| Genetic reagent (M. musculus) | Rosa26FSF-eGFP-L10a | This manuscript | N/A | Rosa26 targeted Flp and flp-dependent eGFP_L10a allele |
| Genetic reagent (M. musculus) | Rosa26RCFL-eGFP-L10a | This manuscript | N/A | Rosa26 targeted Flp-dependent eGFP_L10a allele |
| Software, algorithm | Prism V8 | Graphpad | https://www.graphpad.com/scientific-software/prism/ | |
| Software, algorithm | ImageJ | NIH | https://imagej.nih.gov/ij/download.html |