Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 8;112:104889. doi: 10.1016/j.bioorg.2021.104889

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Elsevier Inc. All rights reserved.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Fig. 1 — Expression and characterization of SARS-CoV-2 M^pro. (a) SDS–PAGE of M^pro. Lane 1, protein marker; lane 2, M^pro before cleavage with HRV 3C-protease; lane 3, M^pro with native C and N termini. (b) the standard curve of fluorescent substrate Mca–AVLQSGFRK(Dnp)K for converting RFU to the amount of the cleaved substrate. (c) Line-weaver Burk plot of 0.1 µM M^pro with various concentrations of substrate.