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Monitoring of fluorescent substrate Mca–AVLQSGFRK(Dnp)K hydrolysis catalyzed by Mpro in the presence of Ebselen 1i and Ebsulfur 2k. The concentrations of substrate and enzyme were 20 and 0.1 μM, respectively. The upper column shows the reaction progression up to 16 min; the middle column shows the progression curves for the first 250 s, which were used for curve fitting to generate the plot shown in the down column. (a), Ebselen 1i; (b), Ebsulfur 2k.
