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The residual activity of Mpro (0.2 µM) incubation with 1 μM Ebsulfur 2k for different period (a), Jump dilution: Mpro was soaked with 1i and 2k (a concentration of 30 × IC50) for 2 h, and then 100-fold diluted with enzymatic substrate solution and monitored for 10 min (b). 0.5% DMSO was as the black control.
