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Structure and mechanism (a) and Gel electrophoresis analysis (b) of the fluorescent reagent Ebs-R labeling/binding Mpro. Lane 1: marker; lane 2: 10 μM purified Mpro; lane 3: 10 μM Mpro and 10 μM Ebs-R incubated; lane 4: 10 μM Mpro and 10 μM Rhodamine B incubated; Right column was observed with UV light irradiating the gel at 365 nm.
