(a) Experimental procedure for the global analysis of the cell surface N-glycoproteome integrating metabolic labeling, copper-free click chemistry, and MS-based proteomics. (b) Different types of sugar analogs, GalNAz, GlcNAz, and ManNAz.
Adapted with permission from Xiao, H., Tang, G. X., & Wu, R. (2016). Site-specific quantification of surface N-glycoproteins in statin-treated liver cells. Analytical Chemistry, 88(6), 3324–3332. Copyright by the American Chemical Society.